Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel.
Identifieur interne : 000235 ( Main/Exploration ); précédent : 000234; suivant : 000236Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel.
Auteurs : K. Loens [Belgique] ; A M Van Loon ; F. Coenjaerts ; Y. Van Aarle ; H. Goossens ; P. Wallace ; E J C. Claas ; M. IevenSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2012.
Descripteurs français
- KwdFr :
- Allemagne, Assurance de la qualité des soins de santé (), Bactéries (isolement et purification), Humains, Infections bactériennes (diagnostic), Infections bactériennes (microbiologie), Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (microbiologie), Infections de l'appareil respiratoire (virologie), Maladies virales (diagnostic), Maladies virales (virologie), Normes de référence, Pays-Bas, Techniques d'amplification d'acides nucléiques (), Techniques d'amplification d'acides nucléiques (normes), Techniques de diagnostic moléculaire (), Techniques de diagnostic moléculaire (normes), Virus (isolement et purification).
- MESH :
- diagnostic : Infections bactériennes, Infections de l'appareil respiratoire, Maladies virales.
- isolement et purification : Bactéries, Virus.
- microbiologie : Infections bactériennes, Infections de l'appareil respiratoire.
- normes : Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire.
- virologie : Infections de l'appareil respiratoire, Maladies virales.
- Allemagne, Assurance de la qualité des soins de santé, Humains, Normes de référence, Pays-Bas, Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire.
- Wicri :
English descriptors
- KwdEn :
- Bacteria (isolation & purification), Bacterial Infections (diagnosis), Bacterial Infections (microbiology), Germany, Humans, Molecular Diagnostic Techniques (methods), Molecular Diagnostic Techniques (standards), Netherlands, Nucleic Acid Amplification Techniques (methods), Nucleic Acid Amplification Techniques (standards), Quality Assurance, Health Care (methods), Reference Standards, Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (microbiology), Respiratory Tract Infections (virology), Virus Diseases (diagnosis), Virus Diseases (virology), Viruses (isolation & purification).
- MESH :
- geographic : Germany, Netherlands.
- diagnosis : Bacterial Infections, Respiratory Tract Infections, Virus Diseases.
- isolation & purification : Bacteria, Viruses.
- methods : Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Quality Assurance, Health Care.
- microbiology : Bacterial Infections, Respiratory Tract Infections.
- standards : Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques.
- virology : Respiratory Tract Infections, Virus Diseases.
- Humans, Reference Standards.
Abstract
An external quality assessment (EQA) panel consisting of a total of 48 samples in bronchoalveolar lavage (BAL) fluid or transport medium was prepared in collaboration with Quality Control for Molecular Diagnostics (QCMD) (www.qcmd.org). The panel was used to assess the proficiency of the three laboratories that would be responsible for examining the 6,000 samples to be collected in the GRACE Network of Excellence (www.grace-lrti.org). The main objective was to decide on the best-performing testing approach for the detection of influenza viruses A and B, parainfluenza virus types 1 to 3, respiratory syncytial virus (RSV), human metapneumovirus, coronavirus, rhinovirus, adenovirus, Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila by nucleic acid amplification techniques (NAATs). Two approaches were chosen: (i) laboratories testing samples using their in-house procedures for extraction and amplification and (ii) laboratories using their in-house amplification procedures on centrally extracted samples. Furthermore, three commercially available multiplex NAAT tests-the ResPlex (Qiagen GmbH, Hilden, Germany), RespiFinder plus (PathoFinder, Maastricht, The Netherlands), and RespiFinder Smart 21 (PathoFinder) tests-were evaluated by examination of the same EQA panel by the manufacturer. No large differences among the 3 laboratories were noticed when the performances of the assays developed in-house in combination with the in-house extraction procedures were compared. Also, the extraction procedure (central versus local) had little effect on performance. However, large differences in amplification efficacy were found between the commercially available tests; acceptable results were obtained by using the PathoFinder assays.
DOI: 10.1128/JCM.00200-11
PubMed: 22170925
Affiliations:
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The panel was used to assess the proficiency of the three laboratories that would be responsible for examining the 6,000 samples to be collected in the GRACE Network of Excellence (www.grace-lrti.org). The main objective was to decide on the best-performing testing approach for the detection of influenza viruses A and B, parainfluenza virus types 1 to 3, respiratory syncytial virus (RSV), human metapneumovirus, coronavirus, rhinovirus, adenovirus, Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila by nucleic acid amplification techniques (NAATs). Two approaches were chosen: (i) laboratories testing samples using their in-house procedures for extraction and amplification and (ii) laboratories using their in-house amplification procedures on centrally extracted samples. Furthermore, three commercially available multiplex NAAT tests-the ResPlex (Qiagen GmbH, Hilden, Germany), RespiFinder plus (PathoFinder, Maastricht, The Netherlands), and RespiFinder Smart 21 (PathoFinder) tests-were evaluated by examination of the same EQA panel by the manufacturer. No large differences among the 3 laboratories were noticed when the performances of the assays developed in-house in combination with the in-house extraction procedures were compared. Also, the extraction procedure (central versus local) had little effect on performance. However, large differences in amplification efficacy were found between the commercially available tests; acceptable results were obtained by using the PathoFinder assays.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22170925</PMID><DateCompleted><Year>2012</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1098-660X</ISSN><JournalIssue CitedMedium="Internet"><Volume>50</Volume><Issue>3</Issue><PubDate><Year>2012</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel.</ArticleTitle><Pagination><MedlinePgn>977-87</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1128/JCM.00200-11</ELocationID><Abstract><AbstractText>An external quality assessment (EQA) panel consisting of a total of 48 samples in bronchoalveolar lavage (BAL) fluid or transport medium was prepared in collaboration with Quality Control for Molecular Diagnostics (QCMD) (www.qcmd.org). The panel was used to assess the proficiency of the three laboratories that would be responsible for examining the 6,000 samples to be collected in the GRACE Network of Excellence (www.grace-lrti.org). The main objective was to decide on the best-performing testing approach for the detection of influenza viruses A and B, parainfluenza virus types 1 to 3, respiratory syncytial virus (RSV), human metapneumovirus, coronavirus, rhinovirus, adenovirus, Chlamydophila pneumoniae, Mycoplasma pneumoniae, and Legionella pneumophila by nucleic acid amplification techniques (NAATs). Two approaches were chosen: (i) laboratories testing samples using their in-house procedures for extraction and amplification and (ii) laboratories using their in-house amplification procedures on centrally extracted samples. Furthermore, three commercially available multiplex NAAT tests-the ResPlex (Qiagen GmbH, Hilden, Germany), RespiFinder plus (PathoFinder, Maastricht, The Netherlands), and RespiFinder Smart 21 (PathoFinder) tests-were evaluated by examination of the same EQA panel by the manufacturer. No large differences among the 3 laboratories were noticed when the performances of the assays developed in-house in combination with the in-house extraction procedures were compared. Also, the extraction procedure (central versus local) had little effect on performance. However, large differences in amplification efficacy were found between the commercially available tests; acceptable results were obtained by using the PathoFinder assays.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Loens</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium. 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Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D016448">Multicenter Study</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>12</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001424" MajorTopicYN="N">Bacterial Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005858" MajorTopicYN="N" Type="Geographic">Germany</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000592" MajorTopicYN="Y">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009426" MajorTopicYN="N" Type="Geographic">Netherlands</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021141" MajorTopicYN="N">Nucleic Acid Amplification Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName><QualifierName UI="Q000592" MajorTopicYN="Y">standards</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011785" MajorTopicYN="N">Quality Assurance, Health Care</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012015" MajorTopicYN="Y">Reference Standards</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014777" MajorTopicYN="N">Virus Diseases</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014780" MajorTopicYN="N">Viruses</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22170925</ArticleId><ArticleId IdType="pii">JCM.00200-11</ArticleId><ArticleId IdType="doi">10.1128/JCM.00200-11</ArticleId><ArticleId IdType="pmc">PMC3295136</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Microbiol Methods. 2003 Oct;55(1):149-53</Citation><ArticleIdList><ArticleId IdType="pubmed">14500006</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Infect. 2011 Dec;17(12):1900-6</Citation><ArticleIdList><ArticleId IdType="pubmed">21707834</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2004 Apr;42(4):1564-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15071005</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2005 Aug 1;41(3):345-51</Citation><ArticleIdList><ArticleId IdType="pubmed">16007532</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2005 Aug;33(4):306-11</Citation><ArticleIdList><ArticleId IdType="pubmed">15994117</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2007 Feb 15;44(4):568-76</Citation><ArticleIdList><ArticleId IdType="pubmed">17243062</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2007 Feb;45(2):421-5</Citation><ArticleIdList><ArticleId IdType="pubmed">17166966</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol Methods. 2008 Jun;73(3):257-62</Citation><ArticleIdList><ArticleId IdType="pubmed">18378345</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 2008 Oct;21(4):716-47</Citation><ArticleIdList><ArticleId IdType="pubmed">18854489</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2010 Jul;82(7):1266-71</Citation><ArticleIdList><ArticleId IdType="pubmed">20513094</ArticleId></ArticleIdList></Reference><Reference><Citation>J Microbiol Methods. 2010 Aug;82(2):131-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20493214</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Clin Microbiol Infect Dis. 2010 Sep;29(9):1055-69</Citation><ArticleIdList><ArticleId IdType="pubmed">20526788</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2010 Oct;48(10):3569-74</Citation><ArticleIdList><ArticleId IdType="pubmed">20660210</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2011 Jan;50(1):42-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21050809</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2004 Mar;70(3):1483-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15006769</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province d'Anvers</li><li>Région flamande</li></region><settlement><li>Anvers</li></settlement><orgName><li>Université d'Anvers</li></orgName></list><tree><noCountry><name sortKey="Claas, E J C" sort="Claas, E J C" uniqKey="Claas E" first="E J C" last="Claas">E J C. Claas</name><name sortKey="Coenjaerts, F" sort="Coenjaerts, F" uniqKey="Coenjaerts F" first="F" last="Coenjaerts">F. Coenjaerts</name><name sortKey="Goossens, H" sort="Goossens, H" uniqKey="Goossens H" first="H" last="Goossens">H. Goossens</name><name sortKey="Ieven, M" sort="Ieven, M" uniqKey="Ieven M" first="M" last="Ieven">M. Ieven</name><name sortKey="Van Aarle, Y" sort="Van Aarle, Y" uniqKey="Van Aarle Y" first="Y" last="Van Aarle">Y. Van Aarle</name><name sortKey="Van Loon, A M" sort="Van Loon, A M" uniqKey="Van Loon A" first="A M" last="Van Loon">A M Van Loon</name><name sortKey="Wallace, P" sort="Wallace, P" uniqKey="Wallace P" first="P" last="Wallace">P. Wallace</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Loens, K" sort="Loens, K" uniqKey="Loens K" first="K" last="Loens">K. Loens</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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